Search results for "Spin column-based nucleic acid purification"

showing 3 items of 3 documents

Comparing analytical methods to detect SARS-CoV-2 in wastewater

2021

Wastewater based epidemiology (WBE) has emerged as a reliable strategy to assess the coronavirus disease 2019 (COVID-19) pandemic. Recent publications suggest that SARS-CoV-2 detection in wastewater is technically feasible; however, many different protocols are available and most of the methods applied have not been properly validated. To this end, different procedures to concentrate and extract inactivated SARS-CoV-2 and surrogates were initially evaluated. Urban wastewater seeded with gamma-irradiated SARS-CoV-2, porcine epidemic diarrhea virus (PEDV), and mengovirus (MgV) was used to test the concentration efficiency of an aluminum-based adsorption-precipitation method and a polyethylene…

Environmental Engineering010504 meteorology & atmospheric sciencesSwineMicrobiologiaWastewater010501 environmental sciences01 natural sciencesArticleWHO World Health OrganizationAigües residuals MicrobiologiaAluminum-based adsorption-precipitationSpin column-based nucleic acid purificationPEG ratioAnimalsHumansEnvironmental ChemistryPandemicsWaste Management and DisposalPEG polyethylene glycol0105 earth and related environmental sciencesDetection limitCOVID-19 Coronavirus disease 2019ChromatographybiologySARS-CoV-2MgV MengovirusWBE wastewater-based epidemiologyChemistryPorcine epidemic diarrhea virusRT-qPCRExtraction (chemistry)COVID-19Contaminationbiology.organism_classificationSalut públicaPollutionEcologiaPolyethylene glycol precipitationSARS-CoV-2 Severe Acute Respiratory Syndrome Coronavirus 2WastewaterRNAEC European CommissionRNA extractionPorcine epidemic diarrhea virusPEDV Porcine Epidemic Diarrhea virusWastewater based epidemiologyScience of The Total Environment
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Complete decontamination and regeneration of DNA purification silica colum

2008

Silica columns are among the most used DNA purification systems, allowing a good yield of high-quality nucleic acids without organic extractions. Silica column regeneration protocols reported up to now to remove DNA traces are time-consuming, and their effectiveness on genomic DNA has not been demonstrated. Here we report a very rapid regeneration procedure that ensures no DNA carryover, independent of its size, without impairing column efficiency. The method takes advantage of the improved DNA removal by low concentrations of Triton X-100.

ChromatographyOctoxynolBiophysicsFungal geneticsSilica decontaminationGenomic DNACell BiologyHuman decontaminationSaccharomyces cerevisiaeDNA separation by silica adsorptionSilicon DioxideBiochemistryDNA extractionPolymerase Chain Reactionchemistry.chemical_compoundgenomic DNAchemistrySpin column-based nucleic acid purificationNucleic acidGenome FungalParticle SizeDNA FungalMolecular BiologyDNAChromatography High Pressure Liquid
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Extraction of β-blockers from urine with a polymeric monolith modified with 1-allyl-3-methylimidazolium chloride in spin column format

2020

Abstract A glycidyl methacrylate-based monolith was modified with imidazolium-based ionic liquid (IL) to be used as stationary phase for solid-phase extraction (SPE). The host monolithic support was prepared by in-situ UV polymerization in spin column format. Two approaches were developed to incorporate the IL into the polymeric monolithic matrix: generation of IL onto the surface monolith, and copolymerization by addition of the IL to the polymerization mixture, which gave the best results. The resulting sorbent materials were morphologically characterized and used for the isolation of five β-blockers from human urine samples. All SPE steps were accomplished by centrifugation, which reduce…

Glycidyl methacrylatePolymersSurface PropertiesAdrenergic beta-Antagonists02 engineering and technology01 natural sciencesHigh-performance liquid chromatographyAnalytical ChemistryMatrix (chemical analysis)chemistry.chemical_compoundSpin column-based nucleic acid purificationHumansSolid phase extractionParticle SizeMonolithDetection limitgeographygeography.geographical_feature_categoryChromatographyChemistrySolid Phase Extraction010401 analytical chemistryExtraction (chemistry)Imidazoles021001 nanoscience & nanotechnology0104 chemical sciencesAllyl CompoundsEpoxy CompoundsMethacrylates0210 nano-technologyTalanta
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